Cloning PCR fragment into the expression vector

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Thu Mar 7 09:44:59 EST 2002


Yes, but the although the poster was using 2 restricion enzymes, she was
filling in the ends to be blunt-- so they would be compatible with each
other.  Consequently, dephosphorylation might help cut down on background
quite a bit in this case.

Mike

>hi.
>forget doing a dephosphorylation when using two REs.theres no need.u might
>be just losing all ur construct through gel purifying.
>ed
>
>
><http://www.biowww.net/forum/read.php?f=1&i=5340&t=5337>

---




More information about the Methods mailing list