Cloning PCR fragment into the expression vector

Michael L. Sullivan mlsulliv at
Thu Mar 7 09:44:59 EST 2002

Yes, but the although the poster was using 2 restricion enzymes, she was
filling in the ends to be blunt-- so they would be compatible with each
other.  Consequently, dephosphorylation might help cut down on background
quite a bit in this case.


>forget doing a dephosphorylation when using two REs.theres no need.u might
>be just losing all ur construct through gel purifying.


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