blurry bands on SDS-PAGE

Emir Khatipov khatipovNO at NOuchicago.edu
Thu Mar 7 11:25:19 EST 2002


> > 3) pH of your
> > sample is 6.8 as that of the stacking buffer... Could your problem be
>
> I'm not convinced that's a problem - I know I've loaded samples which
> have turned the loading buffer yellow,  but the buffering capacity of
> the gel itself meant they ran OK.  *Good*  sigmonster, have a cookie.
>
Agreed. However, as you mentioned, buffering capacity is an issue => depends
on the strength of the buffer in a sample. That's probably not Michael's
case though.







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