Advice on restriction enzyme choices
junk at hgmp.mrc.ac.uk
Fri Mar 8 05:10:42 EST 2002
Historians believe that in newspost
<vuy1yevrej4.fsf at suspiria.ai.mit.edu> on Thu, 7 Mar 2002, Tom Knight
<tk at suspiria.ai.mit.edu> penned the following literary masterpiece:
>The ones currently on my list are: NotI, PstI, SpeI, and XhoI
All good ones to use. Sma, Nar and Nae seem to be ones to avoid plus
anything with single base overhangs, (poorer ligation efficiencies) but
anything else should be fine.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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