DNA extraction from yeast-like fungus

Jennifer Waters jlw92 at email.byu.edu
Fri Mar 8 14:21:35 EST 2002

I need to extract good, clean DNA from Ustilago bullata sporidia (yeast like
in texture and appearance) for use in AFLP reactions.  Currently, I am
grinding the material with N2 but this is extremely difficult and time
consuming.  Has anyone worked with extractions from non-filamentous fungi or
a yeast that can suggest some kind of lysis buffer that would allow the
bypass of grinding (there is no cell wall so the cells should just be able
to be lysed)?  Thanks


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