X-Gal precipitates...?

David L Haviland David.L.Haviland at uth.tmc.edu
Fri Mar 8 17:44:36 EST 2002


----- Original Message -----
From: "Jean-Paul Herman" <herman.jp at jean-roche.univ-mrs.fr>
To: <methods at hgmp.mrc.ac.uk>
Sent: Friday, March 08, 2002 09:04
Subject: X-Gal precipitates...?


> Does anybody know why there is sometimes a crystalline precipitate
> from the X-gal reaction mix as used to reveal b-galactosidase in
> cells? Stock solutions and the mix are fresh, temperature is RT and
> still, sometimes -but not each time.....!- a precipitate forms.

Jean-Paul:

What are you dissolving it in?  DMF?

I'd opt for DMSO, I've had no problems with it and it is safer.  When I make
a BGal solution for cells, I always prewarm the solution that I am adding
the X-gal to.

David

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