DNA extraction from yeast-like fungus

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Sat Mar 9 01:12:18 EST 2002


Jennifer,

Just asked google for curiousity:
http://ww.google.com/search?hl=en&q=Ustilago+bullata+dna

What about this: http://res2.agr.ca/parc-
crapac/english/2summerland/biotechnology/bakkeren/publicationsAbstracts.htm

regards,
Wo

> I need to extract good, clean DNA from Ustilago bullata sporidia (yeast like in
> texture and appearance) for use in AFLP reactions.  Currently, I am grinding
> the material with N2 but this is extremely difficult and time consuming.  Has
> anyone worked with extractions from non-filamentous fungi or a yeast that can
> suggest some kind of lysis buffer that would allow the bypass of grinding
> (there is no cell wall so the cells should just be able to be lysed)?  Thanks
> 
> 
> <http://www.biowww.net/forum/read.php?f=1&i=5345&t=5345>
> 
> 


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Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan

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