blurry bands on SDS-PAGE

Richard P. Grant rpg14 at
Mon Mar 11 03:58:22 EST 2002

In article 
<Pine.SGI.4.33.0203081702111.3122010-100000 at>,
 Michael Witty <mw132 at> wrote:

> and the other way is to use DTT followed by iodoacetate:
>      R-S-S-R  ->  R-SH + HS-R  ->  R-S-CH2-COO- +  -OOC-CH2-S-R

Funny you should say that.  I'm currently using IOA to block a 
(supposedly) active-site cysteine.  Tip:  use DTNB to validate the 


Richard P. Grant  0x5F9559B1                          MRC Lab of Mol Biol
rpg 'at'             New:
       -- People use PCs. They have affairs with their Macs --

More information about the Methods mailing list