blurry bands on SDS-PAGE

Richard P. Grant rpg14 at yahoo.co.uk.invalid
Mon Mar 11 03:58:22 EST 2002


In article 
<Pine.SGI.4.33.0203081702111.3122010-100000 at mole.bio.cam.ac.uk>,
 Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:

> and the other way is to use DTT followed by iodoacetate:
> 
>      R-S-S-R  ->  R-SH + HS-R  ->  R-S-CH2-COO- +  -OOC-CH2-S-R

Funny you should say that.  I'm currently using IOA to block a 
(supposedly) active-site cysteine.  Tip:  use DTNB to validate the 
reaction.

r

-- 
Richard P. Grant  0x5F9559B1                          MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk             New:   http://www.rg-d.com/BioLOG/
       -- People use PCs. They have affairs with their Macs --




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