blurry bands on SDS-PAGE

Michael Witty mw132 at mole.bio.cam.ac.uk
Mon Mar 11 08:03:29 EST 2002


On Mon, 11 Mar 2002, Richard P. Grant wrote:

> In article
> <Pine.SGI.4.33.0203081702111.3122010-100000 at mole.bio.cam.ac.uk>,
>  Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:
>
> > and the other way is to use DTT followed by iodoacetate:
> >
> >      R-S-S-R  ->  R-SH + HS-R  ->  R-S-CH2-COO- +  -OOC-CH2-S-R
>
> Funny you should say that.  I'm currently using IOA to block a
> (supposedly) active-site cysteine.  Tip:  use DTNB to validate the
> reaction.

Richard,
       you must have . . . I mean do you have an idiot proof step by step
proceedure?   Regards, Mike.




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