DNA ligation

David L Haviland David.L.Haviland at uth.tmc.edu
Mon Mar 11 17:40:34 EST 2002


Mike:

> And, just why is everybody so afraid of gel-purifying fragments.  It
> doesn't take that long and can save lots of headaches down the road!

Exactly...  it is very easy to do.   I agree completely and it really
doesnt' take that much time.

We trough vectors and inserts, and for PCR inserts we simply PPT them down
in NaCl and Isoprop, digest, then trough.    We have rarely had problems and
never the need for TA vectors.

David

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