blurry bands on SDS-PAGE

Richard P. Grant rpg14 at yahoo.co.uk.invalid
Tue Mar 12 07:29:51 EST 2002


In article 
<Pine.SGI.4.33.0203111302340.2889393-100000 at mole.bio.cam.ac.uk>,
 Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:

> On Mon, 11 Mar 2002, Richard P. Grant wrote:
> 
> > In article
> > <Pine.SGI.4.33.0203081702111.3122010-100000 at mole.bio.cam.ac.uk>,
> >  Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:
> >
> > > and the other way is to use DTT followed by iodoacetate:
> > >
> > >      R-S-S-R  ->  R-SH + HS-R  ->  R-S-CH2-COO- +  -OOC-CH2-S-R
> >
> > Funny you should say that.  I'm currently using IOA to block a
> > (supposedly) active-site cysteine.  Tip:  use DTNB to validate the
> > reaction.
> 
> Richard,
>        you must have . . . I mean do you have an idiot proof step by step
> proceedure?   Regards, Mike.

Eh yes - let me get back to you on this one.

r

-- 
Richard P. Grant  0x5F9559B1                           MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk             New:   http://www.rg-d.com/BioLOG/
  
  'Yes, but now I can get my arse kicked more rapidly than ever!' - CK




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