DNA ligation

Bertrand Collet b.collet at abdn.ac.uk
Tue Mar 12 09:41:10 EST 2002


Hello !

> And, just why is everybody so afraid of gel-purifying fragments.  It
> doesn't take that long and can save lots of headaches down the road!

Just to confirm: I always extract + purify (Qiagen Gel Extraction Kit) from
agarose gel (ran in 0.5X TBE, EtBr in the gel) and it works very well for all
my subclonings.

Bertrand

--
Dr Bertrand Collet
Fish immunology group
Zoology Department
Tillydrone avenue
Aberdeen AB24 2TZ
Scotland - UK

Tel (office): (+44)(0)(1224)27 3796
Tel (lab): (+44)(0)(1224)27 2870
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