DNA ligation

Bertrand Collet b.collet at abdn.ac.uk
Tue Mar 12 09:41:10 EST 2002

Hello !

> And, just why is everybody so afraid of gel-purifying fragments.  It
> doesn't take that long and can save lots of headaches down the road!

Just to confirm: I always extract + purify (Qiagen Gel Extraction Kit) from
agarose gel (ran in 0.5X TBE, EtBr in the gel) and it works very well for all
my subclonings.


Dr Bertrand Collet
Fish immunology group
Zoology Department
Tillydrone avenue
Aberdeen AB24 2TZ
Scotland - UK

Tel (office): (+44)(0)(1224)27 3796
Tel (lab): (+44)(0)(1224)27 2870
Fax: (+44)(0)(1224)27 2396

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