Problems with compDNA
kknald at hotmail.com
Tue Mar 12 10:57:59 EST 2002
I'm not sure what you mean, DpnI is to my knowledge a restriction enzyme and
it do not distinct between cDNA and the biotinylated DNA strand.... Please
"Michael Witty" <mw132 at mole.bio.cam.ac.uk> skrev i en meddelelse
news:Pine.SGI.4.33.0203121532450.3458448-100000 at mole.bio.cam.ac.uk...
> On Tue, 12 Mar 2002, knud wrote:
> > Hi
> > Do anybody know how to get rid of the complementary DNA strand after
> > upper strand is Biotinylated, and I would be really happy to get rid of
> > non biotinylated complementary strand since it is causing me trouble in
> > DNA:DNA hybridization. Is there somekind of Matrix I can use??? Thanks
> > advance.
> > Mads Hollegaard
> . . . is this where a person should use DpnI? Mike.
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