Problems with compDNA

knud kknald at hotmail.com
Tue Mar 12 10:57:59 EST 2002


Hi Mike
I'm not sure what you mean, DpnI is to my knowledge a restriction enzyme and
it do not distinct between cDNA and the biotinylated DNA strand.... Please
explain. Mads
"Michael Witty" <mw132 at mole.bio.cam.ac.uk> skrev i en meddelelse
news:Pine.SGI.4.33.0203121532450.3458448-100000 at mole.bio.cam.ac.uk...
> On Tue, 12 Mar 2002, knud wrote:
>
> > Hi
> > Do anybody know how to get rid of the complementary DNA strand after
PCR. My
> > upper strand is Biotinylated, and I would be really happy to get rid of
the
> > non biotinylated complementary strand since it is causing me trouble in
my
> > DNA:DNA hybridization. Is there somekind of Matrix I can use??? Thanks
in
> > advance.
> > Mads Hollegaard
>
>   . . . is this where a person should use DpnI?  Mike.
>





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