Problems with compDNA

Alex Brands abbrands at artsci.wustl.edu
Tue Mar 12 11:51:57 EST 2002


On Tue, 12 Mar 2002, knud wrote:

> Hi Mike
> I'm not sure what you mean, DpnI is to my knowledge a restriction enzyme and
> it do not distinct between cDNA and the biotinylated DNA strand.... Please
> explain. Mads

I think you two are talking about different things.  DpnI is methylation
sensitive, and so won't cut PCR product, but will chop the template to
bits.  So, DpnI is useful for getting rid of the template DNA, but you
want to generate single stranded DNA, correct?


> "Michael Witty" <mw132 at mole.bio.cam.ac.uk> skrev i en meddelelse
> news:Pine.SGI.4.33.0203121532450.3458448-100000 at mole.bio.cam.ac.uk...
> > On Tue, 12 Mar 2002, knud wrote:
> >
> > > Hi
> > > Do anybody know how to get rid of the complementary DNA strand after
> PCR. My
> > > upper strand is Biotinylated, and I would be really happy to get rid of
> the
> > > non biotinylated complementary strand since it is causing me trouble in
> my
> > > DNA:DNA hybridization. Is there somekind of Matrix I can use??? Thanks
> in
> > > advance.
> > > Mads Hollegaard
> >
> >   . . . is this where a person should use DpnI?  Mike.
> >
>
>
>

Alex Brands
Washington University









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