radioactive vs. non-radioactive ISH
Paul at genedetect.com
Wed Mar 13 02:10:23 EST 2002
Historically, the benefits of using radioactive in situ hybridization (ISH)
were the superior sensitivity versus non-radioactive methods. More recently
however a number of advances have been made that bring the sensitivity of
non-radioactive ISH to levels similar or greater than radioactive ISH.
For a general read about ISH see http://www.genedetect.com/insitu.htm
These advances include much better detection reagents, notably tyramide
signal amplification (TSA) kits....these kits allow for significant signal
intensification.....if the target gene is not "detected" with one round of
TSA then it is possible to run through another round of TSA to further
amplify the signal. Most major suppliers have their own TSA kits for sale,
like DAKO's GenPoint kit for example.
The other advancement has occurred in probe labeling techniques. For example
our hyperlabeled GreenStar probes.
Put the two together and the sensitivity differences between non-radioactive
versus radioactive ISH become small.
Generally for ISH at the single cell level, non radioactive ISH is the way
to go....for relative quantification...radioactive ISH.
Of course, not having to deal with radioactive substances is a big plus.
Hope this helps
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