Problems with compDNA

knud kknald at hotmail.com
Wed Mar 13 04:48:17 EST 2002


Hi Alex.
That is completly correct,

"Alex Brands" <abbrands at artsci.wustl.edu> skrev i en meddelelse
news:Pine.GSO.4.31.0203121049580.28178-100000 at ascc.artsci.wustl.edu...
> On Tue, 12 Mar 2002, knud wrote:
>
> > Hi Mike
> > I'm not sure what you mean, DpnI is to my knowledge a restriction enzyme
and
> > it do not distinct between cDNA and the biotinylated DNA strand....
Please
> > explain. Mads
>
> I think you two are talking about different things.  DpnI is methylation
> sensitive, and so won't cut PCR product, but will chop the template to
> bits.  So, DpnI is useful for getting rid of the template DNA, but you
> want to generate single stranded DNA, correct?
>
>
> > "Michael Witty" <mw132 at mole.bio.cam.ac.uk> skrev i en meddelelse
> > news:Pine.SGI.4.33.0203121532450.3458448-100000 at mole.bio.cam.ac.uk...
> > > On Tue, 12 Mar 2002, knud wrote:
> > >
> > > > Hi
> > > > Do anybody know how to get rid of the complementary DNA strand after
> > PCR. My
> > > > upper strand is Biotinylated, and I would be really happy to get rid
of
> > the
> > > > non biotinylated complementary strand since it is causing me trouble
in
> > my
> > > > DNA:DNA hybridization. Is there somekind of Matrix I can use???
Thanks
> > in
> > > > advance.
> > > > Mads Hollegaard
> > >
> > >   . . . is this where a person should use DpnI?  Mike.
> > >
> >
> >
> >
>
> Alex Brands
> Washington University
>
>
>
>
>
>





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