Competent cells at OD = 0.94

Peter Ianakiev ianakiev at genome.wi.mit.edu
Wed Mar 13 10:05:38 EST 2002


Hi guys,
Has someone tried to make competent cells at OD600=0.94?
Original paper has been published by Tang, X. et al. 1994 Nucleic acids
Res. 22, 2857-2858.  I wonder if someone reproduced the results.
Any suggestions of making ultra competent (library grade) cells will be
appreciated. Inoue method looks good, but has an  insufficient yield for
our needs.
Thank you for your help
Peter Ianakiev




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