how to make a protein soluble

Min mhkang at facstaff.wisc.edu
Thu Mar 14 14:23:57 EST 2002


Hi, there

I think your protein is expressed as inclusion body.
If you want to resolve protein for SDS-PAGE, 
You can just use 8 M Urea or 6 M Guanidium-HCl in any buffer solution.
Actually, Guanidium-HCl is better than Urea to resolve inclusion body, but
For SDS, I recommned you to use Urea because it is hard for you to load
protein with Gu-HCl into SDS-PAGE.

Good Luck .. 

Min


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