Can I invert labels of LightCycler probes?

Duncan Clark junk at
Fri Mar 15 09:16:56 EST 2002

Historians believe that in newspost <3C90C598.668E0199 at> 
on Thu, 14 Mar 2002, Reimo Tetzner <reimo.tetzner at> 
penned the following literary masterpiece:
>What do you think about inversion of the dye-labels of
>hybridization probes?

You should be able to invert the labels providing you still have a FRET. 
It is no good putting a 5' label on the first probe and a 3' on the 
second, looking left to right.

It should make no difference whether the 3' dye is FAM or LC providing 
the 5' is LC or FAM.

FAM/Cy5 or FAM/Cy5.5 work well.

The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.

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