cloning problem (cont.)

prashanth bobprashanth at rediffmail.com
Sun Mar 17 14:39:27 EST 2002


well jinglan 
you can reverse the orientation of your insert by purifying your insert and
set up a ligation for 2hrs at room temperature with the vector where you
have used for getting the wrong orientation.


<http://www.biowww.net/forum/read.php?f=1&i=5361&t=5318>




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