cloning problem

prashanth bobprashanth at rediffmail.com
Sun Mar 17 14:35:27 EST 2002


hi jinglang liu
if your ligation is not working with double digestion, make sure that you
can linearize your vector first with xhoI, and then cut with NotI and purify
with Geneclean purification technique and make the ligation with the same
ratio.


<http://www.biowww.net/forum/read.php?f=1&i=5359&t=5315>




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