western blotting - transferring a small MW protein

Michael Witty mw132 at mole.bio.cam.ac.uk
Sat Mar 23 10:40:45 EST 2002


> From: john.hines at yale.edu
> Date: Saturday, March 23, 2002 8:35 am
> Subject: western blotting - transferring a small MW protein
>
> > Hey everyone,
> >
> >   This is the first time that I have ever tried to do a western for
> > something so small.  My target protein is 10 kDa.  I'm used to larger
> > targets (40 - 100 kDa).  I'm doing wet transfer in the standard
> > Tris/Glycine/SDS/methanol buffer from out of Harlow and Lane.  Pure
> > nitrocellulose with the .45 micron pore size.

I think there is so much protein to protein variation you must just try!
Mike.




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