PCR template quality??

NM acoustic52 at hotmail.com
Sat Mar 23 18:15:34 EST 2002


How would one go about checking the quality of a DNA template via
electrophoresis prior to using it for a PCR reaction?

What should genomic DNA look like on a gel? Will it even migrate into
the gel if it's supercoiled? If the template is degraded, will there
be an obvious 'laddering' of the sample? How much does one load?

Neal




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