Can Streptavidin bind directly to DNA??
junk at hgmp.mrc.ac.uk
Mon Mar 25 06:06:58 EST 2002
Historians believe that in newspost
<3c9865e3$0$66989$edfadb0f at dspool01.news.tele.dk> on Wed, 20 Mar 2002,
knud <kknald at hotmail.com> penned the following literary masterpiece:
>Is it possible for streptavidin to bind directly to a short DNA oligo, app.
>20 bp long?? Is there a specific sequence for this, if binding is
It won't bind to just any sequence, however it may be that if the 20mer
has the right secondary structure from it's sequence you may well be
able to get a specific sequence that would bind streptavidin.
In general you will need a biotin group on the oligo.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
More information about the Methods