Can Streptavidin bind directly to DNA??

Duncan Clark junk at hgmp.mrc.ac.uk
Mon Mar 25 06:06:58 EST 2002


Historians believe that in newspost 
<3c9865e3$0$66989$edfadb0f at dspool01.news.tele.dk> on Wed, 20 Mar 2002, 
knud <kknald at hotmail.com> penned the following literary masterpiece:
>Is it possible for streptavidin to bind directly to a short DNA oligo, app.
>20 bp long?? Is there a specific sequence for this, if binding is
>possible.....

It won't bind to just any sequence, however it may be that if the 20mer 
has the right secondary structure from it's sequence you may well be 
able to get a specific sequence that would bind streptavidin.

In general you will need a biotin group on the oligo.

Duncan

-- 
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.




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