PCR primer storage?
Michael L. Sullivan
mlsulliv at facstaff.wisc.edu
Mon Mar 25 08:54:58 EST 2002
I like to store oligonucleotides in 10 mM Tris, 0.1 mM EDTA pH 8.0 and I
genearlly resuspend the oligos at 50 pmol/ul. I believe the oligos are
quite stable in this buffer at -20, and the buffer is dilute enough that it
won't affect your PCR reactions.
>What is the best diluent to use to dilute PCR primers? ANy
>advantage/disadvantage to storing them at -70??
Michael L. Sullivan, Ph.D
U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706
(608) 264-5144 Phone
(608) 264-5147 Fax
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