GST Purification

ChenHA hzhen at
Tue Mar 26 04:33:20 EST 2002

Mark Olson wrote:
> Actually, I ran a test sample using a 40kd cut off Millipore
> centrifugal tube/filter. The SDS revealed the GST didn't go through,
> it just stayed in solution with my fusion protein. I guess something
> is preventing the GST from going through the membrane. I wonder if I
> need to use a high salt buffer to spin the sample in.

GST is a dimer.

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