PCR cloning

Netscape User Mika.K.Rantanen at Helsinki.Fi
Tue Mar 26 06:16:47 EST 2002


Hi,
I try to get 100 million clones from a 900 Bp PCR insert. I use two RE:s
at the ends of the PCR fragment (BglII and SalI) but I have obtained
only 1000 clones with RT or 16 degrees ligations. Does anybody know how
to scale-up the number of clones, or is 100 million clones just out of
reach?

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