PCR cloning

Netscape User Mika.K.Rantanen at Helsinki.Fi
Tue Mar 26 06:11:22 EST 2002


Hi,
I have a 900 Bp PCR mixture with variation at six sites. I need 100
million independent expression clones from this PCR, but with two
restriction sites at ends (BglII and SalI) I have only obtained 1000
clones. How could I scale-up the amount of clones? I have done ligations
both at RT and at 16 degrees.
Thanks for any replies, MR

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