purification of small DNA fragment

Marc Busch mgbusch at primate.ucdavis.edu
Tue Mar 26 19:01:13 EST 2002


Hope someone out there can come up with something. I have a small fragment
(~36bp) I want to digest out of a plasmid and recover for subcloning
purposes. Tried a real thick (2.2%) agarose gel but the band was never
visible. So I guess I could go with a TBE polyacrylamide gel and extract
from that. However, I don't have a kit / protocol to do that. Any ideas?
Thanks in advance for any ideas.
                        Marc Busch





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