pKa of amino acid residue in enzyme

Chris chris at
Wed Mar 27 06:27:21 EST 2002


I have a histidine which I believe is acting as a base to abstract a proton
from  a cysteine at the active site of an enzyme I am working on and was
wondering if anyone knew of any ways to measure the pKa of either the
histidine and/or the cysteine (preferably not by NMR) . I do have alanine
mutants of both the cysteine and histidine if those are at all useful in this.

Thanks in advance for your help



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