pKa of amino acid residue in enzyme
wolfsc at ibms.sinica.edu.tw
Wed Mar 27 07:26:53 EST 2002
Just an idea: Could you get the information you need from differences by pI
i.e. by isoelectric focussing?
> I have a histidine which I believe is acting as a base to abstract a proton
> from a cysteine at the active site of an enzyme I am working on and was
> wondering if anyone knew of any ways to measure the pKa of either the histidine
> and/or the cysteine (preferably not by NMR) . I do have alanine mutants of both
> the cysteine and histidine if those are at all useful in this.
> Thanks in advance for your help
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan
More information about the Methods