pKa of amino acid residue in enzyme

Wolfgang Schechinger wolfsc at
Wed Mar 27 07:26:53 EST 2002


Just an idea: Could you get the information you need from differences by pI 
i.e. by isoelectric focussing?


> Hi
> I have a histidine which I believe is acting as a base to abstract a proton
> from  a cysteine at the active site of an enzyme I am working on and was
> wondering if anyone knew of any ways to measure the pKa of either the histidine
> and/or the cysteine (preferably not by NMR) . I do have alanine mutants of both
> the cysteine and histidine if those are at all useful in this.
> Thanks in advance for your help
> Chris
> <>

Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan


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