pKa of amino acid residue in enzyme

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Wed Mar 27 07:26:53 EST 2002


Chris, 

Just an idea: Could you get the information you need from differences by pI 
i.e. by isoelectric focussing?

Regards,
Wo

> Hi
> 
> I have a histidine which I believe is acting as a base to abstract a proton
> from  a cysteine at the active site of an enzyme I am working on and was
> wondering if anyone knew of any ways to measure the pKa of either the histidine
> and/or the cysteine (preferably not by NMR) . I do have alanine mutants of both
> the cysteine and histidine if those are at all useful in this.
> 
> Thanks in advance for your help
> 
> Chris
> 
> 
> <http://www.biowww.net/forum/read.php?f=6&i=984&t=984>
> 
> 


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Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan

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