danielmeb at hotmail.com
Wed Mar 27 11:47:42 EST 2002
i am having problems with cloning a 2.5kb fragment into a c2.1 TOPO vector.
PCR fragments less 1Kb pop in the vector without any problem. i tried to
extend the incubation time to 30min. it still did not work.
do you have any idea? i called invitrogen, they couldn't give any helpful
any tip will be appreciated.
Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp.
More information about the Methods