TOPO cloning

Daniel Mebrahtu danielmeb at hotmail.com
Wed Mar 27 11:47:42 EST 2002


Hi all,

i am having problems with cloning a 2.5kb fragment into a c2.1 TOPO vector. 
PCR fragments less 1Kb pop in the vector without any problem. i tried to 
extend the incubation time to 30min. it still did not work.

do you have any idea? i called invitrogen, they couldn't give any helpful 
suggestions.

any tip will be appreciated.

daniel

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