ABI Instrument file troubles
andren at rom.on.ca
Thu Mar 28 17:48:24 EST 2002
Forgive the cross-posting, I am new here, but we have been having
trouble with our ABI 377. Since having the camera replaced we have had
trouble with our instrument file.
We attempted to create a new one using Data Utility and the Big
Dye standards. However, using this new intrument file we are only
able to call ~450bp of the pGEM plasmid standard. After that the
peaks become fat and blurry. We suspect the standard sample files
used to create the instrument file are not perfect. Even though there
has been no leakage problems (we are using a paper comb, we have tried
the sharktooth comb as well) we are still seeing some irregular
baseline readings of the other three colours with each standard.
Does anyone have any suggestions, links, or ideas that may help?
Your assistance is greatly appreciated.
Andre Ngo email: andren at rom.on.ca
tel:(w) (416) 586-8094
tel:(h) (416) 533-9293
Centre for Biodiversity and Conservation Biology
Royal Ontario Museum
100 Queen's Park
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