Snail cDNA library
Michael L. Sullivan
mlsulliv at facstaff.wisc.edu
Tue May 21 08:55:49 EST 2002
I've made a few cDNA libraries using the ZAP cDNA synthesis kit from
Stratagene (no affiliation). This kit gave me very good results and
I thought was easy to use. Your cDNA ends up being in lambda ZAP,
which is easily converted to pBluescript phagemids. I made only one
modification to their protocol, which was to use a Pharmacia SizeSep
400 spin column for doing the size selection step. The kit calls for
5 ug of polyA RNA as starting material.
I think the kit costs about $1000 US, which includes reagents to make
5 libraries as well as packaging extract.
Hope this helps.
>I need to make cDNA library of a snail. In case you
>have experience in making libraries, please inform me
>how to go about it. How much mRNA will be required to
>begin with? Names of companies providing good kits for
>making libraries. Or any companies which would make it
>and the putative cost. Any other relevant information
>will be highly appreciated.
>Bye for now,
Michael L. Sullivan, Ph.D
U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706
(608) 264-5144 Phone
(608) 264-5147 Fax
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