gel filtration problem

[dustjohn at hotmail.com]

Carol Chihara wrote:

> We are using a Superdex column for protein MWs.  The column has a 
> habit of working well for one or two runs then when it is closed off 
> for a few days or a week it seems to be plugged up.  i.e. we do not 
> get a good flow rate (or almost none at all).  Is there something we 
> don't know?
> Any help will be appreciated.

> -- 
> Carol Chihara, Professor
> Department of Biology
> University of San Francisco
> 2130 Fulton St.
> San Francisco, CA 94117-1080

> ---

Hi Carol--if you visit the Bionet proteins section, there are some questions/answers regarding what you ask...my take on it is that the flow rate is reduced due to bacterial growth or due to aggregated proteins sticking to the resin.
Dustin



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