Real time PCR endogenous controls

j.m.coxhead at ncl.ac.uk j.m.coxhead at ncl.ac.uk
Mon Nov 4 07:05:45 EST 2002


I'm using quantitative real time PCR to investigate human colorectal cell lines during the cell cycle. I'm having difficulty deciding on an appropriate endogenous control. I need a control that is not influenced by the cell cycle but is different to 18S and beta-actin. Any help or advice would be much appreciated


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