lysis buffers, zymography and EDTA

Jen jrobertson1 at sympatico.ca
Mon Nov 4 22:54:26 EST 2002


I use a standard PLC lysis buffer containing EDTA. I'm making whole
cell lysates for zymography. 

(1) I'm assuming that the EDTA would kill the enzymatic activity of
the proteins I'm interested in (bad thing)
(2) can I re-make the lysis buffer and just omit EDTA?





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