Maximum distance between ATG & SP6 promoter

Studio401 rupertyip at
Wed Nov 6 17:05:14 EST 2002

I'd like to express a protein with a cDNA that contains ~180 bases
upstream of the first ATG.  Will cloning this cDNA into an expression
vector (with an SP6 promoter) express protein or is the ATG too far
away from the SP6 promoter?

Does anyone know if there is a maximum distance between the SP6
promoter and the 1st ATG?  Is there are rule of thumb for such things?

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