Lysis buffer for membrane proteins and coimmunoprecipitation

D.K. dk at no.email.thankstospam.net
Fri Nov 8 11:52:03 EST 2002


In article <3DCBCBAC.FE962CB9 at molbio.unizh.ch>, Denise Vogel <denise.vogel at molbio.unizh.ch> wrote:
>I have to isolate a membrane protein from transfected HEK293 cells to do
>a coimmunoprecipitation. I am looking for a lysis buffer which contains
>reagents which do not denature the antibody. Could somebody help me?
>Thank you!
>

At 1-2% practically any non-ionic detegergent, mild ionic ones like CHAPS
and cholate, and in really difficult cases when something is very difficult to 
solubilize, up to 0.1-0.2% SDS. 

Octyl-glucoside, Tween-20, Triton X-100 are the usual first choice 
fore what you are trying to do.

DK



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