Lysis buffer for membrane proteins and coimmunoprecipitation

Wolfgang Schechinger hubahopp at gmx.de
Fri Nov 8 14:29:17 EST 2002


Denise,

you might leave out reducing agents like BME, DTT or DTE as well as ionic
detergents e.g. SDS.

Regards,

Wo


At 03:35 PM 2002/11/8 +0100, Denise Vogel wrote:
>I have to isolate a membrane protein from transfected HEK293 cells to do
>a coimmunoprecipitation. I am looking for a lysis buffer which contains
>reagents which do not denature the antibody. Could somebody help me?
>Thank you!
>
>

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