external control for RT-PCR

Tuncay Baubec bass.tard at gmx.at
Sat Nov 9 15:14:29 EST 2002


well try using gapdh.
always got best results with that

with best regards,
tuncay


"Chunxin Wang" <cxwang at wam.umd.edu> schrieb im Newsbeitrag
news:Pine.GSO.4.21.0211081157010.15184-100000 at rac1.wam.umd.edu...
> Hi all,
>
> We're currently using actin as the external control for RT-PCR. For some
> reasons, we couldn't get stable results. It worked well sometimes and then
> didn't work at all. Or it preferentially gets amplified in a certain
> of tissues.
>
> Any suggestions on which gene is a better control? How about tubulin?
>
> Thanks!
>
> Black
>
>
> ---





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