Dnase digestion after Invitro transcription

Sergio add at yahoo.com
Mon Nov 11 08:08:47 EST 2002

> Do you have any idea how to improve the DNase digestion?

I use to add MgSO4 50mM to improve the DNAse step (37ºC 1h30' to 2h). Maybe it's 
worthy to try it.


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