Lysis buffer for membrane proteins and coimmunoprecipitation

Duncan Clark junk at hgmp.mrc.ac.uk
Mon Nov 11 08:02:15 EST 2002


Historians believe that in newspost 
<c09b237b.0211081511.dcbac59 at posting.google.com> on Fri, 8 Nov 2002, 
John Ladasky <ladasky at my-deja.com> penned the following literary 
masterpiece:
>*PMSF stock solution: 50 mM PMSF in 2-propanol.  Stable for at least
>three months at room temperature.

Providing the propanol is very dry?

I remember 20 years or so ago cadging dry propanol from the protease lab 
next door specifically for PMSF. Can't remember what they used to stick 
in the Winchester to keep it dry - some sort of brown pelleted stuff  - 
I know, not much use without a name :(

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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