Can a linearized Plasmid DNA ligate inside the E.coli?
lakshmanan1 at llnl.gov
Mon Nov 11 14:20:47 EST 2002
I have digested my plasmid with Xho1 and verified with agarose gel
and gel purified a single band using Qiagen gel purification kit.
When I transformed this DNA into DH10B, by electrophoration, I got
several colonies. FYI, I do not dephosphorilate my vector. I think
the linearized vector is ligating inside the E.coli cells. Could
this be possible? I would appreciate your thought and experience on
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