Can a linearized Plasmid DNA ligate inside the E.coli?

Wolfgang Schechinger hubahopp at gmx.de
Tue Nov 12 04:28:33 EST 2002



Mike, 

are there any ExoV(minus) strains?

Wolfgang

>I have always thought "yes", but it is inefficient.  Is it exonuclease V
>which causes the low efficiency of linear DNA transformation in E coli?  I
>also would appreciate expert opinion.  Mike.
>
>

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