Can a linearized Plasmid DNA ligate inside the E.coli?

Wolfgang Schechinger hubahopp at
Tue Nov 12 04:28:33 EST 2002


are there any ExoV(minus) strains?


>I have always thought "yes", but it is inefficient.  Is it exonuclease V
>which causes the low efficiency of linear DNA transformation in E coli?  I
>also would appreciate expert opinion.  Mike.


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