external control for RT-PCR

[Tim]

Hi Chunxin,

You don't mention the source of your RNA.  Some folks have demonstrated some
variability in HKG from different sources.  You might like to take a look
at: http://genomebiology.com/2002/3/7/research/0034.1.  Maybe this will
help.

Tim Spahlinger

"Chunxin Wang" <cxwang at wam.umd.edu> wrote in message
news:Pine.GSO.4.21.0211081157010.15184-100000 at rac1.wam.umd.edu...
> Hi all,
>
> We're currently using actin as the external control for RT-PCR. For some
> reasons, we couldn't get stable results. It worked well sometimes and then
> didn't work at all. Or it preferentially gets amplified in a certain
> of tissues.
>
> Any suggestions on which gene is a better control? How about tubulin?
>
> Thanks!
>
> Black
>
>
> ---