color of loading dye

van wjvan at
Wed Nov 13 02:14:50 EST 2002

Hi ... this is a stupid question but I need some helps here.  :-)

I tried to make some loading dye for the PCR gel.

I had tried 0.05% - 0.25% ( w/v ) bromphenol blue in 50% glycerol ( or in
Sucrose ).  The color is not blue but dark brown/orange.

Do I need to adjust pH or add EDTA?   Any clue?

Thanks !!


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