color of loading dye

Henk Veldman H.Veldman-2 at neuro.azu.nl
Wed Nov 13 02:52:48 EST 2002


van schreef:
> 
> Hi ... this is a stupid question but I need some helps here.  :-)
> 
> I tried to make some loading dye for the PCR gel.
> 
> I had tried 0.05% - 0.25% ( w/v ) bromphenol blue in 50% glycerol ( or in
> Sucrose ).  The color is not blue but dark brown/orange.
> 
> Do I need to adjust pH or add EDTA?   Any clue?
> 

50 % glycerol or sucrose in TAE-buffer (the same buffer you use in your
gel and as a running-buffer).

Henk



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