color of loading dye
H.Veldman-2 at neuro.azu.nl
Wed Nov 13 02:52:48 EST 2002
> Hi ... this is a stupid question but I need some helps here. :-)
> I tried to make some loading dye for the PCR gel.
> I had tried 0.05% - 0.25% ( w/v ) bromphenol blue in 50% glycerol ( or in
> Sucrose ). The color is not blue but dark brown/orange.
> Do I need to adjust pH or add EDTA? Any clue?
50 % glycerol or sucrose in TAE-buffer (the same buffer you use in your
gel and as a running-buffer).
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