color of loading dye

Wolfgang Schechinger hubahopp at gmx.de
Wed Nov 13 04:18:06 EST 2002


Van,

it's probably a pH issue. You might add solid tris-BASE until the color
changes to blue. Or could it be that the BP concentration is too high? 

Regards,

Wo


At 07:14 AM 2002/11/13 GMT, van wrote:
>Hi ... this is a stupid question but I need some helps here.  :-)
>
>I tried to make some loading dye for the PCR gel.
>
>I had tried 0.05% - 0.25% ( w/v ) bromphenol blue in 50% glycerol ( or in
>Sucrose ).  The color is not blue but dark brown/orange.
>
>Do I need to adjust pH or add EDTA?   Any clue?
>
>Thanks !!
>
>Van
>
>
>

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