color of loading dye
hubahopp at gmx.de
Wed Nov 13 04:18:06 EST 2002
it's probably a pH issue. You might add solid tris-BASE until the color
changes to blue. Or could it be that the BP concentration is too high?
At 07:14 AM 2002/11/13 GMT, van wrote:
>Hi ... this is a stupid question but I need some helps here. :-)
>I tried to make some loading dye for the PCR gel.
>I had tried 0.05% - 0.25% ( w/v ) bromphenol blue in 50% glycerol ( or in
>Sucrose ). The color is not blue but dark brown/orange.
>Do I need to adjust pH or add EDTA? Any clue?
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