color of loading dye
Mark_Dowton at uow.edu.au
Wed Nov 13 18:34:38 EST 2002
It's definitely a pH problem. We add around 40-50 ul of 1 M Tris per ml of
dye to turn it from yellow to blue. This will also increase the solubility of
Wolfgang Schechinger wrote:
> it's probably a pH issue. You might add solid tris-BASE until the color
> changes to blue. Or could it be that the BP concentration is too high?
> At 07:14 AM 2002/11/13 GMT, van wrote:
> >Hi ... this is a stupid question but I need some helps here. :-)
> >I tried to make some loading dye for the PCR gel.
> >I had tried 0.05% - 0.25% ( w/v ) bromphenol blue in 50% glycerol ( or in
> >Sucrose ). The color is not blue but dark brown/orange.
> >Do I need to adjust pH or add EDTA? Any clue?
> >Thanks !!
Institute for Conservation Biology
Wollongong NSW 2522
mailto:mdowton at uow.edu.au
Centre for Evolutionary Biology & Biodiversity
Dept Evironmental Biology
Adelaide University SA 5005
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