external control for RT-PCR

[L. Lintott]

Here is another site to look at, with several papers on use of
housekeeping genes in real time RT-PCR, but they would apply to any
RT-PCR
http://www.wzw.tum.de/gene-quantification/index.shtml

They all talk about problems with using 'house-keeping genes' and that
maybe 18s is a better choice.

cheers
LL

Chunxin Wang wrote:
> 
> Hi all,
> 
> We're currently using actin as the external control for RT-PCR. For some
> reasons, we couldn't get stable results. It worked well sometimes and then
> didn't work at all. Or it preferentially gets amplified in a certain
> of tissues.
> 
> Any suggestions on which gene is a better control? How about tubulin?
> 
> Thanks!
> 
> Black
> 
> ---

-- 
Lauri Lintott
Research Technician
Molecular Biology of Potato Pathogens

Agriculture and Agri-Food Canada
business e-mail: lintottl at em.agr.ca
home e-mail: llintott at shaw.ca